how to calculation 39 11. Use these values and the protein concentration complet
ID: 151550 • Letter: H
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how to calculation 39
11. Use these values and the protein concentration complete the following table. determined in the previous section to Parameter Value Units Notes 1 Dilution factor of (N/A) enzyme used in the assay 2 Actual concentration mg/mL Pooled fractions Of 20 L protein used 10, concentration/dilution factor to initiate reaction 3 Actual concentration of protein used in cuvette mg/mL Row 2 x (0.02/1) 4 Slope1 AA/min Taken from your plot 5 Slope 2 AA/min AA/min 6 Average slope Average of row 4 and row 5 Change in A per min 7 Change in /min (Row 6/6220) x 10 concentration of NADH (per minute 8 Amount of NADH mol/min Row 7 x 103 consumed in 1 minute 9 Specific activity (after anion exchange chromatography) Aumol/min/mg Row 8/Row 3 or units/mgExplanation / Answer
3- take avg. of absorbance of 10sec and multiply it with0.02
4- take avg of 30 seconds absorbance
5-take avg. of 40 seconds absorbance, 6-- take sum of avgof 30sec. and 40 sec. absorbance and divide it by 2
7- take avg. of 50 secabsorbance divide it by 6220 and multiply it with 1000000
8- take avg. absorbance of 60 sec and multiply it with 1000
9- divide avg. absorbance of 70 sec. by 400 seconds
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