You have been asked to measure the partition coefficient of drug candidates. All
ID: 512378 • Letter: Y
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You have been asked to measure the partition coefficient of drug candidates. All the procedures you have found require that the drug candidate absorb light in the UV-visible range when dissolved in water. What is the absorbance used for? The absorbance will be used to ensure that the drug is not phototoxic to people. The absorbance will be used to detect impurities. The absorbance will be used to measure the concentration of the drug. The absorbance will be used to identify the drug. You have found that the pain killer, acetaminophen, has an absorbance in the UV range of light and want to use it as a test compound for your measurement technique. The suggested procedure involves the use of unfamiliar glassware, a separatory funnel. Look up a separatory funnel and describe how it will be used to reach the equilibrium of drug solubility in the two solvents.Explanation / Answer
2. The absorbace is used for the absorbance will be used to measure the concentration of the drug.
The drug has the absorbance in the UV region which can be used to estimate the concentration of the drug. Using Beer Lambert equation, we can estimate the concentration of the substance (drug).
3. We can use two solvents, one of the them being water and other phase (hexane or any other solvent, based on the polarity required) is organic in nature in a separating funnel. So when we take the drug in aqueous phase and equilbrate with the organic phase, there is a distriburiton of the drug in two phase which we define the partition coefficent (The concentration of the drug in the organic phase to the concentration in the aqueous phase). We can estimate the drug in the aqueous and organic phase by measuring the concentation of the drug by using UV spectrophotmeter. Hence we can estimate the equilibrium concentration for a given pair of solvent for the drug.
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