4) (15 pts) a. What is the order in which acetone, acetamide and 1,2-dichloroeth

Question

4) (15 pts) a. What is the order in which acetone, acetamide and 1,2-dichloroethane would be eluted from an HPLC column packed with C18 hydrocarbon bonded to the siloxane backbone material?

b. Give an example of a mobile phase solvent/solvent system that can be used with the column above.

c. Roughly sketch a chromatogram (label the peaks) that would be obtained if the mixture in part a is run through a column with –C2H4CN functional group attached to the siloxane backbone and n-Hexane as the mobile phase.

Explanation / Answer

(a)

elute

Acetamide-first

acetone-second

1,2 dichloroethane-last

(b)

Any polar solvent or mixtures of those: water, methanol, acetonitrile, etc

(c)

A chromatogram showing 1, 2 dichloroethane eluting first then acetone and last is acetamide

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