Section PRE-LAB QUESTIONS EXPERIMENT Redox Reactions: Detecting Traces of Blood

Question

Section PRE-LAB QUESTIONS EXPERIMENT Redox Reactions: Detecting Traces of Blood l 25 1. Plant peroxidases, proteins that contain heme and catalyze the oxidation of organic compounds by hydrogen peroxide, can interfere with tests for blood or hemoglobin. Heating samples to 100 "C for 20 minutes can deactivate plant peroxidases. Why should you not be surprised that these plant enzymes can interfere with tests for blood? 2. Find MSDS for phenolthphalein and leucomalachite green on the Internet. What health concerns are provided? What precautions should you take in handling these substances? Copyright 2017 Pearson Education, Inc.

Explanation / Answer

1. Plant peroxidases are hemoproteins that have the prosthetic group ferriprotoporphyrin. Plant peroxidases have a much higher enzymatic activity than Hb. Because of differences in the Apo proteins of the two molecules the Hb Apo protein does not survive transit through the gut.

2.

MSDS of Phenolphthalein

Product Name: Phenolphthalein TS

Flammability of the Product: Flammable.

Auto-Ignition Temperature: The lowest known value is 363°C (685.4°F) (Ethyl alcohol 200 Proof)

Physical state and appearance: Liquid.

Color: Colorless.

pH (1% soln/water): Neutral.

Boiling Point: The lowest known value is 78.5°C (173.3°F)

Melting Point: May start to solidify at -114.1°C (-173.4°F

Critical Temperature: The lowest known value is 243°C (469.4°F)

Specific Gravity: Weighted average: 0.8

Vapor Pressure: The highest known value is 5.7 kPa (@ 20°C)

Vapor Density: The highest known value is 1.59 (Air = 1)

Volatility: Not available.

Odor Threshold: The highest known value is 100 ppm (Ethyl alcohol 200 Proof)

Water/Oil Dist. Coeff.: Not available.

Ionicity (in Water): Not available.

Dispersion Properties: See solubility in water, methanol, diethyl ether, acetone.

Solubility:

Easily soluble in cold water, hot water, methanol, diethyl ether. Soluble in acetone.

Hazards Identification

Potential Acute Health Effects: Hazardous in case of skin contact (irritant), of eye contact (irritant), of ingestion, . Slightly hazardous in case of skin contact(permeator). Non-corrosive for skin. Non-corrosive to the eyes. Non-corrosive for lungs.

Potential Chronic Health Effects: CARCINOGENIC EFFECTS: Classified PROVEN by State of California Proposition 65 [Ethyl alcohol 200 Proof]. ClassifiedA4 (Not classifiable for human or animal.) by ACGIH [Ethyl alcohol 200 Proof].

Toxic Effects on Humans

Hazardous in case of skin contact (irritant), of ingestion, of inhalation. Slightly hazardous in case of skin contact (permeator) May affect genetic material (mutagenic) Causes adverse reproductive effects and birth defects (teratogenic) , based onmoderate to heavy consumption. May cause cancer based on animal data. Human: passes through the placenta, excreted inmaternal milk. (Ethyl alcohol 200 Proof)

Acute potential health effects: Skin: causes skin irritation Eyes: causes eye irritation Ingestion: May affect behavior.Moderately toxic and narcotic in high concentrations. May affect metabolism, gastrointestinal tract, blood, liver. May affectrespiratory tract, cardiovascular and urinary systems. Experimentaly tumorigen. Inhalation: May cause irritation of therespiratory tract, headache, drowsiness, nausea, narcosis.

MSDS OF Leucomalachite green

Physical State: Powder
Appearance: almost white
Odor: none reported
pH: Not available.
Vapor Pressure: Not available.
Vapor Density: Not available.
Evaporation Rate:Not available.
Viscosity: Not available.
Boiling Point: Not available.
Freezing/Melting Point:100.00 - 102.00 deg C
Decomposition Temperature:Not available.
Solubility: slightly soluble
Specific Gravity/Density:Not available.
Molecular Formula:C23H26N2
Molecular Weight:330.46

Eye: No information regarding eye irritation and other potential effects was found.
Skin: No information regarding skin irritation and other potential effects was found.
Ingestion: The toxicological properties of this substance have not been fully investigated.
Inhalation: The toxicological properties of this substance have not been fully investigated.

HAZARDS IDENTIFICATION
EMERGENCY OVERVIEW
Light sensitive.Air sensitive.The toxicological properties of this material have not been fully investigated.
Potential Health Effects
Eye:
No information regarding eye irritation and other potential effects was found.
Skin:
No information regarding skin irritation and other potential effects was found.
Ingestion:
The toxicological properties of this substance have not been fully investigated.
Inhalation:
The toxicological properties of this substance have not been fully investigated.

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