5.7 Enzyme conversion An immobilised enzyme process is used in an ice-cream fact

Question

5.7 Enzyme conversion An immobilised enzyme process is used in an ice-cream factory to hydrolyse lactose (c12H22011) to glucose (CoH1206 and galactose (C6H1206 C12H22011 H2O C6H12O6 C6H12O6 Gel beads containing B-galactosidase are packed into a column reactor; 2500 kg of lactose enters the reactor per day as a 10% solution in water at 25°C. The reactor operates at steady state and 32°C; all of the lactose is converted. Because the heat of reaction for enzyme conversions is not as great as for cell culture, sensible heat changes and heats of mixing cannot be ignored. 2. MATERIAL AND ENERGY BALANCES

Explanation / Answer

the standard heat of the reaction Hrxn, also called standard enthalpy is diference between the heat of combusytion of reactants and products and measures heat of the reaction under standard conditions i.e. at 25°C and 1 atm pressure.Hc° (glucose)= -2805.0 Hc° (galactose)= -2805.7;

Hrxn= (1 mol* -2805.0+1 mol* -2805.7)- (1 mol* -5652.5 + 0)

Hrxn= -5651.8 kJ and is exothermic reaction needs cooling as the reaction mixture becomes very hot.

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