BACTERIAL GROWTH AND CONTROLLING BACTERIAL GROWTH 1. Based upon your results, is
ID: 49887 • Letter: B
Question
BACTERIAL GROWTH AND CONTROLLING BACTERIAL GROWTH
1. Based upon your results, is your bacteria sample a strict aerobe, strict anaerobe, facultative anaerobe, or microaerophile? Why? – Look at your results from OF Glucose and OF Glucose with oil test.
2. Did the bacteria grow in the tryptone or malonate media? Why or why not? – Remember, you can tell if they grew if culture was turbid (cloudy).
3. For the media used in this portion of the experiment, are the media complex or synthetic? Are the media selective, differential, both, or neither? Identify the carbon and nitrogen source for each medium. – Read more about what these terms mean starting on page 178 in your book right below Figure 6.11. Look up each media with the M? button. Remember, nutrient broth is a complex media, mineral salts is a defined media.
4. How many possible bacteria were remaining after conducting these biochemical tests?
5. Using the information in the Identification matrix, what additional tests might help you to identify the bacteria in your case study? –Look at the View Identification Matrix under the View menu. What tests would you perform? Look for ones that have a mix of + and – because they would help distinguish between organisms.
Explanation / Answer
1.Bacteria can be classified into many categories based on the presence and absence of oxygen. If it grows strictly in the presence of oxygen then it is called aerobic. if it doesnot require oxygen but can also grow in its presence, then it is called anaerobic. If the oxygen requirement is optional then facultative anaerobes. If cannot grow in the presence of oxygen obligative anaerobes.
Hence if the bacteria grows in OF glucose and cannot grow in the OF glucose with oil then it is strictly aerobic. If it doesnot grow in the OF glucose then it is strictly anaerobic. Grows in both the conditions facultative anaerobic.
2. The growth of the bacteris can be determined by the changing of the broth media to turbid due to the increase in turbidity. Tryptone is a soruce of amino acids and malonate is asource of carbohydrates both can able to grow bacteria
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