BACTERIAL GROWTH - GENERATION TIME CALCULATION ASSIGNMENT ? Using the growth cur

Question

BACTERIAL GROWTH - GENERATION TIME CALCULATION ASSIGNMENT

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Using the growth curve shown and the indicated points on the graph, calculate:

Part A - The number of generations for the culture using the abosorbance or optical density scale, n = :

Part B - The generation time (Gt) for the culture using the abosorbance or optical density scale points is:

Part C - The number of generations for the culture using the viable cell count scale, n = :

Part D - The generation time (Gt) for the culture using the viable count scale points is:

Part E - The generation time calculation (Gt) that you would trust to really assess the generation time for this culture would be?

9.32 generations 4.63 generations 6.19 generations 2 generations 3.10 generations 1011 1 0 1.5 CFUs /ml absorbance 1010 10 I x 10 CFU/mL 10 3 EsszzEs:zz.1 01 10 7 0.02 2 001 2 x 10' CFU/mL 4 time Chours 7

Explanation / Answer

Part A: 6.19 generations

PartB. : 29 mins/ generations

Part C : 9.32 generations

PartD: 30 mins/ generations

Part E: option B - (considering the viable cells )

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