The enzyme-catalyzed conversion of a substrate at 25C has a Michaelis constant o
ID: 498222 • Letter: T
Question
The enzyme-catalyzed conversion of a substrate at 25C has a Michaelis constant of 0.034 mol L-1 . The rate of the reaction is 8.64 x 10-3 mol L-1 s -1 when the substrate concentration is 0.290 mol L-1 . What is the maximum velocity of this enzymolysis?
5. 6. The enzyme-catalyzed conversion of a substrate at 250C has a Michaelis constant of 0.034 mol L 1. The rate of the reaction is 8.64 x 10 3 mol LT s when the substrate concentration is 0.290 mol L What is the maximum velocity of this enzymolysis?Explanation / Answer
. What is the maximum velocity of this enzymolysis?
Dp/dt =K[E]
K=Kb[S] /Km +[S]
V= Kb[S] [E]/Km +[S]
Kb[S] ([E] =(Km +[S] /[S] r
=(0.034+0.290/0.290) *8.64 x 10^-3
=9.652 X10^-3
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