1.List in words or indicate in a drawing the important features of a plasmid vec
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Question
1.List in words or indicate in a drawing the important features of a plasmid vector that are required to clone a gene. Explain the purpose of each feature.
2.What role do restriction enzymes have in nature?
3.Using your understanding of evolution, why would bacteria retain a gene that gives them resistance to antibiotics? How is the existence of bacteria with antibiotic resistance affecting medicine today?
4.Bacteria, sea anemones, and humans seem, on the surface, to be very different organisms. Explain how a gene from humans or a sea anemone can be expressed in bacteria to make a product never before made in bacteria.
5.Due to a mishap in the lab, bacteria carrying a plasmid with a kanamycin- resistant gene and bacteria carrying a plasmid with an ampicillin-resistant gene were accidentally mixed together. Design an experiment that will allow you to sort out the two kinds of bacteria. (Hint: Make sure that you do not kill off one of the kinds of bacteria you are trying to sort out!)
Explanation / Answer
1.)
Features of a plasmid vector
Ori = origin of replication
Ara-C = binds to promoter therefore we tend to get transcription of sequence of interest.
Amp-R = selectable marker
RFP = red fluorescent protein which is a gene of interest
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