If 1 ml of a sample is added to 4 ml of diluent, what is the dilution factor? A

Question

If 1 ml of a sample is added to 4 ml of diluent, what is the dilution factor? A serum sample is outside the linear range of the analyzer for an analyte. A 1:2 ratio of serum to diluent is obtained, and the sample is reanalyzed. What factor would be needed to multiply the result of the diluted sample in order to obtain the correct concentration of the analyte? A serum triglyceride sample is diluted to 1/10 with a result = 75 mg/dL. What is the patient's actual triglyceride level? A 10-fold serial dilution is performed, with a final dilution of 1/10,000. The first dilution in the series in tube >>1 is 1/10. If the original concentration had been 35,000 ng/dL, what would the concentration be in tube #4? If a serial dilution is performed and a final concentration of the sample is 0.296 mg/dL, with a dilution factor of 1200, what is the original concentration?

Explanation / Answer

1]

1 ml of a sample is added to 4 ml of diluent what is the dilution factor

The final volume is equal to the sample volume PLUS the diluent volume:
1ml + 4 ml = 5ml

The dilution factor is equal to the final volume divided by the sample volume:
5ml/ 1ml = 1:5 dilution

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