Using the information below, provide a recipe for 20 ml of 10X loading dye. How

Question

Using the information below, provide a recipe for 20 ml of 10X loading dye. How much of each of the two dyes will you weigh out? How much of the glycerol solution that you made in question #2 will you be using?

When adding your DNA samples to the agarose gel, you will need to add loading dye to the samples. Loading dye contains:

•       glycerol, which helps the samples sink into the sample wells of the gel; and

•       tracking dyes so that you have a way of estimating how far the samples have travelled through the gel during electrophoresis.

The recipe for 6X loading dye is as follows:

•       0.25% bromophenol blue (w/v)

•       0.25% xylene cyanol (w/v)

•       30% glycerol in water (v/v)

(Important tip: Remember that you have already made 30ml of a 50% stock solution of glycerol. I recommend determining the concentration of glycerol required for 10X loading dye, then figuring out how much of your 50% glycerol stock will be needed to make 20ml of 10X loading dye.)

Explanation / Answer

The recipe for 6X (means 6 times concentrated) loading dye is as follows:

•       0.25% bromophenol blue (w/v)

•       0.25% xylene cyanol (w/v)

•       30% glycerol in water (v/v)

The recipe for 1X (means10 times concentrated) loading dye would therefore have,

•       0.25% bromophenol blue (w/v)

•       0.25% xylene cyanol (w/v)

•       50% glycerol in water (v/v)

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