1.) What color would a gram negative be if you accidentally swapped the order of
ID: 48792 • Letter: 1
Question
1.) What color would a gram negative be if you accidentally swapped the order of safranin and crystal violet and why?2.) What color would an L-form gram positive bacterium be? Explain referencing structures/molecules.
3.) Gram positive cells stain purple and gram negative cells stain pink. In the simple stain, are the purple cells necessarily gram positive? Why or why not?
These are questions from my microbiology course. Please help me! I'm so confused. 1.) What color would a gram negative be if you accidentally swapped the order of safranin and crystal violet and why?
2.) What color would an L-form gram positive bacterium be? Explain referencing structures/molecules.
3.) Gram positive cells stain purple and gram negative cells stain pink. In the simple stain, are the purple cells necessarily gram positive? Why or why not?
These are questions from my microbiology course. Please help me! I'm so confused.
2.) What color would an L-form gram positive bacterium be? Explain referencing structures/molecules.
3.) Gram positive cells stain purple and gram negative cells stain pink. In the simple stain, are the purple cells necessarily gram positive? Why or why not?
These are questions from my microbiology course. Please help me! I'm so confused.
Explanation / Answer
1) We will get some blue bacteria under the and no pink ones, a false result in case if there were gram negative bacteria.
Gram's method involves washing with ethanol to remove the crystal violet from gram negative bacteria, If crystal violet was used earlier, however if the steps were reversed the safranin would be washed out of both negatives and positives and the crystal violet would stick in both cell types, hence the blue color
2) L-forms can be developed from a gram positive or gram negative bacteria, but all L forms stain gram negative, because they lack a cell wall.
3) In a simple staining all the cells are purple color irrespective of whether they are gram positive or gram negative.
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