Why not use 100% diethyl ether as the eluant in this experiment? What problem wi

Question

Why not use 100% diethyl ether as the eluant in this experiment? What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis? What will be the result of applying too much compound to a TLC plate? What will be the appearance of a TLC plate if a solvent of too low polarity is used for the development? too high polarity? What would be the effect of collecting larger fractions eluting from the silica column in this experiment? Once the chromatographic column has been prepared, why is it important to allow the level of the liquid in the column to drop to the level of the silica gel before applying the compound to be separated? Arrange the following in order of increasing Rf on thin-layer chromatography: acetic acid, acetaldehyde, 2-octanone, decane, and 1-butanol. Order of Solute Migration on Chromatography Fastest Alkanes Alkyl halides Alkenes Dienes Aromatic hydrocarbons Aromatic halides Ethers Esters Ketones Aldehydes Amines Alcohols Phenols Carboxylic acids Sulfonic acids Slowest

Explanation / Answer

3. Due to high polarity, diethyl ether can not differentiate between the different component of the mixture. All the components will move together

4. If solvent front is higher then your spots will spread unpredictably over your TLC plate and your results will be unreliable.

5. If the too much compound is applied, spots will be big resulting in overlapping spots

6. too low polarity - spots will not move - no separation

high polarity - spots will move together - no effective separation

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