1. Consider a tobacco plant cell that was able to express a toxin that was letha

Question

1. Consider a tobacco plant cell that was able to express a toxin that was lethal to insects and was resistant to the antibiotic kanamycin. Which of the following genes would this cell contain?

Select one:

a. lacZ

b. Bt

c. Bt and neo+

d. Bt, neo+ and Lacz

e. neo+

2. Which of the following represents an appropriate cloning vector for cloning a gene into a bacterial cell?

Select one:

a. Ti plasmid

b. Agrobacterium tumefaciens

c. Plasmid

d. YAC

e. lacZ

3. Suppose you have previously located and cloned a human gene on chromosome You now believe there is an important disease-related gene nearby on the same chromosomE. What technique might you employ to locate the disease-related gene?

Select one:

a. Chromosome walking

b. Site-directed mutagenesis

c. RNAi

d. DNA fingerprinting

e. None of the above could be used in this situation.

4. Which of the following would be most appropriate for cloning a gene that is 325 kb in size?

Select one:

a. BAC

b. Cosmid

c. Plasmid

d. Phage lambda

e. Yeast phage

Explanation / Answer

1. d.Bt, neo+ and Lacz. because bt is a toxin, neomycine phosphotransferase confers resistance to kanamycin and lacZ is required of efficient transfer of the gene.

2.C. plasmid. beacuse it requires plasmid with Ori (origin of replication, ORF and selection marker for cloning into bacteri, agrobacterium containing Ti plasmid for transfer of genes into plants and YAC is specialized for cloning in yeast)

3.a. Chromosome walking. this technique is employed when exact position of a gene on a chromosome is not known. chromoromes distances on nearly few Kb to 1Mb on genetic map.

4.BAC. beacuse. the order from smallest to largest insert for cloning: Plasmid(15Kb)<Phage lambda(11Kb)<cosmid(45Kb)<BAC(350Kb)<YAC(3000kb)

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