1. Consider a pool of mutations which are identified as A, B, C, and D in a hypo

Question

1. Consider a pool of mutations which are identified as A, B, C, and D in a hypothetical inducible operon under negative regulation and the gene for its associated regulatory protein. The operon expresses two genes that produce enzyme 1 and enzyme 2, respectively. Given the consequences of each mutation (below), identify ALL of the following locations in which it is possible for the single mutation to be found:

(Hint 3: Mutations of structural genes result in the production of defective protein products).

Possible Locations
A). gene encoding enzyme 1     B). gene encoding enzyme 2                      C). promoter
D). repressor allosteric site        E). repressor DNA binding domain            F). operator
           

Mutation     Enzyme 1               Enzyme 2            ALL Possible Locations_____________          
    A              inducible                           no activity           gene encoding enzyme 2
     B             constitutive           constitutive      ______________ or ________________
     C             no activity             inducible           _________________
     D            no activity              no activity         ______________ or ________________

Explanation / Answer

MUTATION ENZYME 1 ENZYME 2 ALL POSSIBLE LOCATIONS A INDUCIBLE NO ACTIVITY GENE ENCODING ENZYME 2 B CONSTITUTIVE CONSTITUTIVE REPRESSOR ALLOSTERIC SITE OR OPERATOR C NO ACTIVITY INDUCIBLE GENE ENCODING ENZYME 1 D NO ACTIVITY NO ACTIVITY PROMOTER OR REPRESSOR DNA BINDING DOMAIN

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