X ncorrect X ncorrect X ncorrect X Question 14 of 23 Map General Chemistry 4th E

Question

X ncorrect X ncorrect X ncorrect X Question 14 of 23 Map General Chemistry 4th Edition University Science Books Rock presented by Sapling Leaming The data to the right were collected for the bovine carbonic anhydrase-catalyzed reaction described by [co2 (mmol. L-1) R (Amol. L 1. srt) 28.74 1.25 H (aq) +HCO, (aq) 250 48.61 80.28 5.00 Use these values to determine the value of KM, the Michaelis- 155.59 20.00 Menten constant. Answer the four parts below. a) If you were to plot this data to graphically determine R and KM using a Lineweaver-Burk plot, what would you plot for the following data? lf you would plot the original data, enter the original value in the corresponding blank J mmol. L i CO graph as graph as Number Number 250 48.61 L. s mmol L mmol Number Number -e L. s mmol L mmol 155.59 20.00

Explanation / Answer

(a)

Plot 2.50 as 1/2.50 = 0.4

Plot 20.00 as 0.05

Plot 48.61 as 0.020572

Plot 155.59 as 0.006427

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If you plot the data a strainght line will be obtained. The equation for this straight line is :y = 0.0377x + 0.0049

slope = 0.0377

intercept = 0.0049

y intercept = 1/Vmax = 0.0049

or, Rmax = 1/0.0049 = 204.082 mmol/L/s

slope = Km/Vax = 0.0377

Km = 0.0377 * 204.082 mmol/L/s = 7.694 mmol

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