pling order the steps of the Southern blot procedure as they would be applied to

Question

pling order the steps of the Southern blot procedure as they would be applied to DNA fingerprinting analysis. First step Incubate membrane with radioactively labeled ssDNA probe For step 1, keep in mind that DNA fragments are fractionated by gel electrophoresis. Separate DNA fragments by gel electrophoresis. Transfer ssDNA to a nylon or nitrocellulose membrane. Denature DNA by soaking gel in NaOH. Cleave DNA using restriction endonucleases. Place film onto membrane to produce autoradiogram. Last step

Explanation / Answer

The following is the order of Southern blotting procedure.

1. Cleave DNA using endonucleases.

2. Separate fragments by gel electrophoresis.

3. Denature DNA by soaking gel in NaOH.

4. Transfer ssDNA to a nylon or nitrocellulose membrane.

5. Incubate membrane with raioactively labelled ssDNA probe.

6. Place film into membrane to produce autoradiogram.

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