plesae help with what you can Why must you use a pencil and not ink or felt-tip
ID: 481316 • Letter: P
Question
plesae help with what you can
Why must you use a pencil and not ink or felt-tip pen, to mark on the origin of the spots of the amino acid preparations? In this experiment, suppose you forgot to mark the position of the solvent front when you tested the hydrolysate of aspartame against the other amino acids. Is it still possible to determine how many amino acids were present in the hydrolysate? Explain your answer. Could you still identify what those amino acids were? Explain your answer. What can you not do? There are polarity and molecular weight differences between aspartic acid and phenylalanine Aspartic acid has a polar, acidic side chain and a smaller molecular weight has a non polar side and a larger molecular weight. Based on the R _f values obtained for these two you these two amino acids in the solvent employed, which amino acid migrated faster and which of Properties influenced the rate of migration? From your experiment, is there any evidence that the aspartame in the Diet Coca-Cola* sample showed any hydrolysis into the two amino acids?Explanation / Answer
Q1.
The pencil is essentially Cabon as graphite (solid, nonpolar)
The ink is most likely a mixture of polar/sluble solvents which may interact with the sample, so avoid this
Q2
a)
Most likely yes, since they will stil split, even though you will not be able to determine which is which one
b)
Not likely, since there is no order, and you do not know the affinity/retentions
c)
Try again, it is most likely easier to repeat and perform the experiment correctly.
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