Next, how much of each stock (below) plus water do you use for 500 mL of the buf

Question

Next, how much of each stock (below) plus water do you use for 500 mL of the buffer recipe above? Choose units so that your answer is measurable- e.g. not 0.05 L or 50,000 uL, but 50 mL. Show your work. 5 M NaCl _______ 1 M Tris, pH 8.0 ______ 0.5 M EDTA ______ distilled water _______ The label on the bottle of Drug X in the clinic states that the concentration is 4 grams per 5 mL. How many mg would be contained in 200 mu L? You are asked to give a dog his daily dose of insulin. The required dose is 0.5 U/kg every 24 hours. The dog weighs 44 lbs (2.2 lbs = 1 kg). The bottle of insulin you find in the refrigerator states the concentration of insulin to be 40 U/ml. How many units will you administer to the animal? How many mL of the drug will you inject per dose? If a solution of Pentothal is a 7% solution: How many mg are there in 100 mL of the solution? If we use a dose of 1 mL/5lb, how many mL would be required for a 20 kg dog? How many mg would we be giving the 20 kg dog? Finish replicating the following strand of DNA (top), as would be done by DNA

Explanation / Answer

4. Drug X concentration = 4 g/5 ml

So, in 200 uL = 0.2 ml, we have = 4 g x 0.2 ml/5 ml = 0.16 g = 160 mg

5. Dog weighs = 44 lbs = 44/2.2 = 20 kg

a. units to administer to the animal = 20 kg x 0.5 U/1 kg = 10 units

b. ml of drug to inject per dose = 0.5/40 = 0.0125 ml = 12.5 uL

6. Pentothal solution = 7% means 7 g in 100 ml

a. mg are there in 100 ml = 7,000 mg

b. 20 kg = 20 x 2.2 = 44 lb

5 lb require 1 ml, so 44 lb require dose = 8.8 ml

c. mg given to the 20 kg dog = 7000 x 8.8/100 = 616 mg

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