1) Below are several steps in isolating cDNA that encodes protein X found in the

Question

1) Below are several steps in isolating cDNA that encodes protein X found in the brain tissue. From the choices available (a,b,c or d), select the sequential order of steps (1-4) that would be successful:

1. transform the cloned cDNAs into bacteria

2. Prepare cDNAs and insert them into a plasmid vector

3. Isolate mRNA from brain tissue

4. Do colony hybridization to screen transformed bacterial colonies for ones that hybridize to prove it derived from gene X

a) 1,2,3,4

b) 3,2,1,4

c) 3,1,2,4

d) 4,3,2,1

Explanation / Answer

C

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