30 If a fermentation tube is inoculated with an organism after suitable incubati
ID: 48179 • Letter: 3
Question
30 If a fermentation tube is inoculated with an organism after suitable incubation it is observed that there is no change in the indicator, no gas, and no turbidity, which of the conclusions would be correct c The organism does not attack the arbohy drate in question. B. The organisms not grow in this medium. C. The organism does not produce gas in this medium. B. This organism produces neither acid nor gas in this medium. E. None of these 31. Phenol red (Mark all possible answers) A. Turns deeper red in a basic solution B. Turns yellow in an acid solution C. Turns yellow in a basic solution 32. A general enzymatic equation is? [E-S-C] e none of a d choices 33 Exo enzymes carry out their reactions in which location? a. outside the cell b. cytoplasm c mitochondrion d. Golgi complex e nuclear region 34. All of the following are enzymes except: a. lactose b. dehydrogenase c. lipase d. peptidase e catalase 35. If a sample a mixture of different organisms which contains of the following of would allow growth of only one type medium a rich nutrient b. selective medium. c. differential medium d. nutrient agar e. none of a-d choices
Explanation / Answer
30. B
The absence of gas or change in the color of the medium can be attributed to the production of gas or acid by the microbe. but the absence of turbidity in the medium suggests that the the organisms did not grow in the medium.
31. A and B. Phenol red turns red in a basic solution and turns yellow in a acidic solution.
32. C. The enzyme reacts with the substrate to form enzyme substrate complex. This then finally forms the product leaving the enzyme free.
33. A. outside the cell. The term exo indicates outside. these enzymes are produced inside the cell and work outside the cell.
34.A. Lactose. Lactose is a carbohydrate found in milk. The other options given are all enzymes.
35. B. Selective media selects for the growth of a specific organism. Nutrient agar enables the growth of many organisms while differential medium is useful in the differentiation between two organisms
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