wild type 5\'AAA AGT CCA TCA CTT AAT GCC 3\' mutant 5\'AAA GTC CAT CAC TTA ATG G

ID: 4782 • Letter: W

Question

wild type 5'AAA AGT CCA TCA CTT AAT GCC 3' mutant 5'AAA GTC CAT CAC TTA ATG GCC3' a) where are the + and - mutations in the mutant DNA? b) what alterations in amino acids occurred in the doublemutant, which produces wild-type plaques? Show both the wild-typeand mutant amino acid sequence. wild type 5'AAA AGT CCA TCA CTT AAT GCC 3' mutant 5'AAA GTC CAT CAC TTA ATG GCC3' a) where are the + and - mutations in the mutant DNA? b) what alterations in amino acids occurred in the doublemutant, which produces wild-type plaques? Show both the wild-typeand mutant amino acid sequence.

Explanation / Answer

TTT Phe (F)
TTC "
TTA Leu (L)
TTG "

TCT Ser (S)
TCC "
TCA "
TCG "

TAT Tyr (Y)
TAC
TAA Ter
TAG Ter

TGT Cys (C)
TGC
TGA Ter
TGG Trp (W)

CTT Leu (L)
CTC "
CTA "
CTG "

CCT Pro (P)
CCC "
CCA "
CCG "

CAT His (H)
CAC "
CAA Gln (Q)
CAG "

CGT Arg (R)
CGC "
CGA "
CGG "

ATT Ile (I)
ATC "
ATA "
ATG Met (M)

ACT Thr (T)
ACC "
ACA "
ACG "

AAT Asn (N)
AAC "
AAA Lys (K)
AAG "

AGT Ser (S)
AGC "
AGA Arg (R)
AGG "

GTT Val (V)
GTC "
GTA "
GTG "

GCT Ala (A)
GCC "
GCA "
GCG "

GAT Asp (D)
GAC "
GAA Glu (E)
GAG "

GGT Gly (G)
GGC "
GGA "
GGG "

Wild type 5'AAA AGT CCA TCA CTT AAT GCC3'

Lys Ser Pro Ser Leu Asn Ala

Mutant 5'AAA GTC CAT CAC TTA ATG GCC 3'

Lys Val His His leu Met Ala

deletion at 4th position