View/perform/read ALL THREE of the following prior to answering the questions. h

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View/perform/read ALL THREE of the following prior to answering the questions.

http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/micro10.swf::Steps in Cloning a Gene (Links to an external site.)

http://www.discoverbiotech.com/wiki/-/wiki/Main/Applications of Cloning (Links to an external site.)

http://www.wiley.com/college/boyer/0470003790/animations/cloning/cloning.htm (Links to an external site.)

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From the list below, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I.              Transform bacteria with recombinant DNA molecule
II.           Cut the plasmid DNA using restriction enzymes
III.         Extract plasmid DNA from bacterial cells
IV.          Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments
V.            Use ligase to seal plasmid DNA to nonplasmid DNA

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Plasmids (or vectors) are important in biotechnology because they are

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Plasmids are put into bacterial cells by

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Restriction enzymes usually

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After combining DNA fragments in a cloning experiment, ___ is used to covalently join the DNA segments.

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It is theoretically possible for a gene from any organism to function in any other organism. Why is this possible?

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Assume that you are trying to insert a gene into a plasmid and someone gives you a DNA sample cut with restriction enzyme X. The gene you wish to insert from the given sample has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to

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Which of the following is/are false in regard to expression plasmids (also called expression vectors)?

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What is NOT a potential problem(s) associated with using bacteria containing a cloned eukaryotic gene (e.g. a human gene) to produce a functional protein?

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Cloning allows for production of proteins in much larger amounts than occurs in the cells from which the gene is isolated.

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Question 111 pts

Gene cloning is used to do all of the following except

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In your

Explanation / Answer

Question nos. are not given. I don't know which question has what no. I am answering all of them....

1. III, II, IV, V, I

2. Plasmids (or vectors) are important in biotechnology because they are a vehicle for the insertion of recombinant DNA into bacteria.

3. Plasmids are put into bacterial cells by transformation.

4. Restriction enzymes make staggered cuts at specific sequences in DNA in both donor DNA and plasmid.

5. After combining DNA fragments in a cloning experiment, DNA ligase is used to covalently join the DNA segments.

6. It is theoretically possible for a gene from any organism to function in any other organism. Why is this possible? All organisms have the same genetic code.

7. cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme.

8. They are the first plasmid type used to clone a gene- This statement is false. The first plasmid type used to clone a gene is pBR322.

9. All of the above are potential problems.

10. True

11. Gene cloning is used to do all of the following except perform gene therapy.

12. To prevent contaminating bacteria from growing.

13. associated activity

14. all of the above

15.The restriction enzyme used to digest the DNA from the patient cut in the middle of the resistance gene

16. The bacteria will grow in the ampicillin broth and the nutrient broth.

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