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Li Watch On × Codons- × How to M: × Amazing C x Facebook x 0 Renae Chmx E Etsy-Abo × LuckyAA c ×. Old Dormir Chegg.comx fi Dwww.saplinglearning.com/ibiscms/mod/ibis/view.php?id=1714686 :: Apps J.Rward Barnes & 3 PINCH me F V Virginia CommonHe "Microsoft Office 2010 Housing & Residenc Members Affect3D-Girlfriend. ons- anacristina ree sa.. Other bookmarks Logout ? Help sapling learning Sapling Learning My Assignment Jump to... Old Dominion University-CHEM 441 -Summer15-LEE Activities and Due Dates Chs DNA Technologies Resources o 6/8/2015 1 1 :00 PM 69.7/100 O 6/5/2015 01:22 AM ! Calculator-Periodic Table Attempts Score Print Available From:5/29/2015 06:00 PM Due Date: Points Possible: 100 Grade Category: Graded Question 11 of 12 6/8/2015 11:00 PM Ma DNA samples from four individuals were cleaved with the same restriction endonuclease. The DNA fragments were separated by gel electrophoresis, transferred to a membrane, and hybridized with a DNA probe complement (see image below). -12 kb I10 kb to a region between sites C and D 8 kb The image of the blot (on right) shows the labeled DNA bands and molecular weight (MW) markers. The lane labels I, II, I, and IV correspond to individuals,, I, and IV You can check your answers. You can view solutions when you complete or give up on any question You can keep trying to answer each question until you get it right or give up. You lose 5% of the points available to each answer in your question for each incorrect attempt at that 6 kb 5 kb Assume that fragments such as C-D and C-E reclly resolved in this gel s A-B is 4 kb. B-c"is 1 kb. C-D is kb, and D-E is 650 bp. Fragment sizes are as follows: Individual I has five cleavage sites (A, B, C, D, and E) for the restriction endonuclease 10 Individual I DNA homologous to probe D E OleTextbook OHelp With This Topic O Web Help &Videos; O Technical Support and Bug Reports 12 Which individual has a point mutation(s) that eliminates... restriction site C only? restriction sites B and C? restriction site A onl Previous ) GiveUp & View Solution (2) Check Answer"Next Exit Hint Copyright 2011-2015 sapllng Learning Inc. 152 about us |careers partnersprlvacy pollicy terms of use contact us help

Explanation / Answer

In the graph there are 4 individuals I , II , III and IV

A probe that corresponds to DNA segment not cut by restriction enzyme produces a single band but if there are similar sequences then more than one band may be there.

For I and III individual we see bands for 5 kb weight marker hence point mutation eliminates restriction site C only are I and III

Restriction sites B and C will be II individual with 6kb weight markers

Restriction site A only will be individual IV with highest weight marker 10kb

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