Lewis gave the following assignment to John. I am working on a sample of Nucleic

Question

Lewis gave the following assignment to John. I am working on a sample of Nucleic Acid, but I didn't have enough time to complete the analysis and computation of the nitrogen bases of this sample. The only thing I was able to complete and count is the percentage of the thymine (T) bases which was 17.2% of all the bases of the sample I want to know: Whether this sample of nucleic acid is ONA or RNA molecule and why? The accurate percentage of the reset of the nitrogen bases in the sample adenine (A, ) guanine (G) & cytosine (C). How can we ensure that the percentages are really accurate? I really need this information and data for my meeting on Monday morning at 7.00 AM. Remember. John, no excuses can be accepted. Good luck.

Explanation / Answer

1) DNA as RNA doesn't contain thymine

2) As there is 17.2% as it always pairs with adenine, Therefore adenine is 17.2%. Remaining cytosine and guanine will account for 100-2(17.2) = 65.6 as they form pairs Therefore each of two will account for 32.8%. Chargaff's rule will help in accuracy

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