Suppose you isolated an nonapeptide from biochemistry lab student\'s blood. Afte

Question

Suppose you isolated an nonapeptide from biochemistry lab student's blood. After getting it absolutely pure you acid hydrolyzed it to find 0.4 mu moles each of R, E. H, M, S. T. C. Y, I. This indicates that the peptide contains only one: Glutamate, Tryptophan, Almandine, Thiamine, Glutamine. Reaction of the peptide (question 23) with FDNB produced a DNP-product after acid hydrolysis containing an sulfhydryl R-group. This indicates that: C terminal is Glycine, N terminal is Cysteine, N terminal is Tyrosine, C terminal is Threonine, C terminal is Tyrosine or Glycine. Treatment of the nonapeptide (question 23) with CNBr produced a tetrapeptide containing the terminal amino acid and a pentapeptide. The pentapeptide after one round of the Edman proceed produced a product that had a aliphatic R group. This means that the pentapeptide has. N-terminal I. N-terminal S. C-terminal E. N-terminal H. C-terminal M. The tetrapeptide (question 25) at pH 6 had a charge of -0.5. This indicates that the tetrapept could be: TEHM. THEM. CHEM. CRHM. YRME.

Explanation / Answer

One letter abbreviations used for the amino acids:

R:Arginine

E:Glutamic acid

H:histidine

M:methionine

S:serine

T:Threonine

C:Cysteine

Y:tyrosine

I:isoleucine

thiamine is vitamin B1

Answer: E:glutamate

Acid hydrolysis converts the glutamate to glutamic acid.

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