OXIDATIVE PHOSPHORYLATION. 1. When pure reduced cytochrome c is added to careful

Question

OXIDATIVE PHOSPHORYLATION.

1. When pure reduced cytochrome c is added to carefully prepared mitochondria along with ADP, Pi, antimycin A, and oxygen, the cytochrome c becomes oxidized, and ATP is formed, with a P/O ration approaching 1.0. a) indicate the probable flow of elecrons in this system. b) why was antimycin A added? c) what does this experiment tell you about thw location of coupling sites for oxidative phosphorylation? d) write a balanced equation for the overall reaction ( including cyt c oxidation and ATP synthesis) e) Calculate delta G for the above reaction, using Enot values from table 14.1 and a delta G valu for ATP hydrolysis of -32.2 kj/mol.

E is the standard potential at pH 7 and 25 degree c, n is the number of electrons transfered, and each potentials is for the partial reaction written as follows: nt + ne- reductant

Explanation / Answer

1a. Lactate and ferredoxin is the source of electrons which flows through a series of intermediate compounds generated during the reactions of Kreb

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