BLAST homework: http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE
ID: 45972 • Letter: B
Question
BLAST homework:
http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome The sequence is:
ATGTTTTCCGGTGGCGGCGGCCCGCTGTCCCCCGGAGGAAAGTCGGCGGC
CAGGGCGGCGTCCGGGTTTTTTGCGCCCGCCGGCCCTCGCGGAGCCGGCC
GGGGACCCCCGCCTTGCTTGAGGCAAAACTTTTACAACCCCTACCTCGCC
CCAGTCGGGACGCAACAGAAGCCGACCGGGCCAACCCAGCGCCATACGTA
CTATAGCGAATGCGATGAATTTCGATTCATCGCCCCGCGGGTGCTGGACG
AGGATGCCCCCCCGGAGAAGCGCGCCGGGGTGCACGACGGTCACCTCAAG
CGCGCCCCCAAGGTGTACTGCGGGGGGGACGAGCGCGACGTCCTCCGCGT
CGGGTCGGGCGGCTTCTGGCCGCGGCGCTCGCGCCTGTGGGGCGGCGTGG
ACCACGCCCCGGCGGGGTTCAACCCCACCGTCACCGTCTTTCACGTGTAC
GACATCCTGGAGAACGTGGAGCACGCGTAC The Homework is:
1. How long is the sequence that was used to search the data base?
Would this be the query leng
2. What is the most likely identity of this sequence? What data supports this conclusion? IT would be a herpes virus, and i don't know what data supports this
3. What organism is the most likely source for the sequence (note, if the sequence if viral, list the virus, not the host)? A virus
4. What is the common name for this organism (note, if the sequence if viral, list the virus, not the host)? I dont know thwat common name besides a herpes virus.
5. What phylum (use family for viruses) contains this organism? I dont know what this is
6. What is the accession number for the best-matching sequence? What is the asscession number?
7. Is this sequence expressed? How do you know?
8. If your sequence is expressed, where is it expressed?
9. If there is a specific time during development when this gene is expressed?
Explanation / Answer
1. Query Length: 480
2. Human herpesvirus 1 strain E12.
3. Herpes virus.
4. HSV - Herpes simplex virus.
5. Herpesviridae.
6. Human herpesvirus 1 strain E12: Max score- 887, total score - 887, query cover - 100%, E value -0.0, identity - 100% and accession number - HM585501.2
7. Since the sequence query cover is 100% and identity is 100%, this sequence is expressed.
8. The sequence is expressed in the genome of herpes virus.
9. May be during assembly of virus particle.
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