Given the following restriction map of a cloned 10 kb piece of DNA, what size fr

Question

Given the following restriction map of a cloned 10 kb piece of DNA, what size fragments would you see after digesting this linear DNA fragment with each of the enzymes or combinations of enzymes listed? (1) EcoRI, (2) BamHI, (3) EcoRI + HindIII, (4) BamHI + PstI, and (5) EcoRI + BamHI.

10. Given the following restriction map of a cloned 10 kb piece of DNA, what size fragments would you see after digesting this linear DNA fragment with each of the enzymes or combinations of enzymes listed? (1) EcoRI, (2) BamHI, (3) EcoRI + HindIII, (4) BamHI+PstI, and (5) EcoRI BamHI. CO E HHEB E HH E B 1.5 0.6 1.0 1.2 2.1 1.9 1.7 kb

Explanation / Answer

Restriction enzymes are the endonuclease enzymes that cut the DNA into fragments of varying lengths. Eacg restriction enzyme cuts the DNA at the specific sequence.

1) DNA fragment obtained by he cut of EcoRI: 1.5kb

2) DNA fragment obtained by BamHI: 2.1kb

3) DNA fragment obtained by EcoRI +HindIII: 1.0+0.6= 1.6kb

4) DNA fragment obtained by BamHI + PstI: 2.1+ 1.9 =4.0kb

5) DNA fragment obtained by EcoRI + BamHI = 1.2kb

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