FACS analysis can be used to: ( select all answers that apply ) A. separate T ce

Question

FACS analysis can be used to: (select all answers that apply)

A.

separate T cells from other types of white blood cells.

B.

quantitate cellular DNA content.

C.

sort cells based on which phase of the cell cycle they are in.

D.

induce the expression of target genes.

E.

transfect animal cells with viral vectors.

A.

separate T cells from other types of white blood cells.

B.

quantitate cellular DNA content.

C.

sort cells based on which phase of the cell cycle they are in.

D.

induce the expression of target genes.

E.

transfect animal cells with viral vectors.

Explanation / Answer

Flow cytometry (FACS analysis) is an analytical method used in the evaluation of cell membrane proteins, intracellular proteins, peptides and DNA. Antigen-antibody reaction is the basic mechanism employe in FACS analysis, the antibodies are fluorescently labelled. Flow cytometry quantification is carried out with intercalating color labels.

Thus, the correct options are,

A. Separate T cells from other type of white blood cells.

B. Quantitate cellular DNA content.

C. sort cells based on which phase of the cell cycle they are in.

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