FACS analysis can be used to: ( select all answers that apply ) A. separate T ce

Question

FACS analysis can be used to: (select all answers that apply)

A.

separate T cells from other types of white blood cells.

B.

quantitate cellular DNA content.

C.

sort cells based on which phase of the cell cycle they are in.

D.

induce the expression of target genes.

E.

transfect animal cells with viral vectors.

A.

separate T cells from other types of white blood cells.

B.

quantitate cellular DNA content.

C.

sort cells based on which phase of the cell cycle they are in.

D.

induce the expression of target genes.

E.

transfect animal cells with viral vectors.

Explanation / Answer

C. sort cells based on which phase of the cell cycle they are in

Explanation

In multicellular organisms, all the cells are identical in their DNA but the proteins vary tremendously. Therefore, it would be very useful if we could separate cells that are phenotypically different from each other. In addition, it would be great to know how many cells expressed proteins of interest, and how much of this protein they expressed. Fluorescence Activated Cell Sorting (FACS) is a method that can accomplish all these goals.

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