1. BCA Assay a) what is this technique used for? b) how does it work? c) how do

ID: 42407 • Letter: 1

Question

1. BCA Assay

a) what is this technique used for?

b) how does it work?

c) how do you determine your sample concentration?

2) Principles of mass sprectrometry (MALDI-TOF)

d) what are the main components of mass spectrometry and what do they do?

e) how does maldi work?

1) why was the development of this technology necessary?

2) what is the matrix and what is the role?

f) how does TOF work?

1) what part of the mass spectrometer is it consider?

2) what does it measure?

3) wich ions reach the detector first?

BCA assay is used to find out the total concentration of protein in a sample.

the principle relies on 2 reactions :

1. peptide bonds reduce Cu++ ----- Cu + , the amount of Cu+ reduced is proportional to the amount of protein present .

2. 2 molecuules of bicinchoninic acid cchelate with each Cu ion forming a purple colored product.

3. sample concentation is ffound by measuring the absorbance and the puuting the value obtaineed on the standard graph of amino acids

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