Molecular and Cellular Biology practice questions I missed class and I don\'t kn

Question

Molecular and Cellular Biology practice questions

I missed class and I don't know how to do the questions, so I chose some questions I think are good bases and will help me do the rest of them:

1) Explain how do you make 500mL of 0.50M Sucrose solution?

2) You want to make 100mL of a 1M fructose solution and our stock concentration is 5M. How do you do this?

3) You want to make 50mL of a 12mM sucrose and our stock concentration is 0.30M. How do you do this?

4) You ordered 500mL of a 50X TAE Stock solution and it has just arrived. You want to make 300mL of a 1X TAE solution. How do you do this?

Explanation / Answer

Explain how do you make 500mL of 0.50M Sucrose solution?

answer , the number of moles of solute present in one liter of the solven is called molarity . moles of solute / liters of solution so to acluculate , i molar of sucrose contain 342 g of sugar for one liter of solution , can be caluculated by Vl Mmol/lt = number of moles of sucrose present in that that 500 ml

500m[ 1lt/1000ml} [0.50 /500 ml ]= 0.5*0.001=0.0005 mol of glucose mix it with 500ml of water

100mL of a 1M fructose solution and our stock concentration is 5M.

c1v1=c2v2 , where c is concentrations of solution   that is 1 and 5 m and v is the volume ,

v2= c1v1/c2 = 1*100/5=20ml volume   you should take .from the stock

500mL of a 50X TAE Stock solution and it has just arrived. You want to make 300mL of a 1X TAE solution.

concentrated (50x) stock solution of TAEmolecular weight isa 242 g (FW = 121.14) and dissolving in approximately 750 mL deionized water. Carefully add 57.1 ml glacial acid acid and 100 mL of 0.5 M EDTA (pH 8.0) and adjust the solution to a final volume of 1 L. then this stock is diluted by 50 ml to get 1x concentration with deionized water .it will give the exact concentartion

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