Vibrio fischeri is a bioluminescent bacterium that lives in the light organ of t
ID: 40922 • Letter: V
Question
Vibrio fischeri is a bioluminescent bacterium that lives in the light organ of the Hawaiian bobtail squid Euprymna scolopes. V. fischeri has the lux operon which includes the promoter (luxP), the operator (luxO), and eight genes that encode proteins involved in light production. The lux operon is regulated by a protein encoded by the luxR gene and a small molecule called AHL. When the concentration of AHL is low, the lux operon is off but when AHL is high, the lux operon is expressed and the bacteria produce light. You are studying Vibrio fischeri mutants in order to better understand regulation of the lux operon. You collect the following data: Is this negative inducible, negative repressible, positive inducible or positive repressible?Explanation / Answer
positive repressible.
Since, AHL allows the expression of lux operon. & it is seen that when R- is there, there is no glow. thus, it can be said that AHL binds to lux R+ (repressor). when lux R is bound by the AHL and thus inhibits the repressor from binding to the operon. Thus, allows lux expression. light is produced.
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