From this diagram, address the following questions. 1. Analysis of the cloning v
ID: 39651 • Letter: F
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From this diagram, address the following questions. 1. Analysis of the cloning vector. First you point out the plasmid vector: pCK1::lac2 What is the function of each labeled section Now will each section be important in your cloning experiment? 1) 2) 3) 4) 5) 2. Review of the cloning process a. During which step in the diagram would DNA ligase be used (1, 2, 3, 4, or 5)? b. During which step in the diagram would a restriction endonuclease be used? c. During which step in the diagram would the ampicillin resistance gene be important? d. Which step represents the transformation of a bacterial cell? e. What must be added to the growth media to ensure expression of the pmoCAB operon? 3. Genetic exchange can occur naturally between bacteria by three different mechanisms. Once in the cow, each of these mechanisms could result in the transfer of pCK1::mmo from the recombinant E. coli to a bacterium resident in the cow ru Explain how each mechanism would occur.Explanation / Answer
1.
1. ligation, 2. PCR amplification 3. restriction digestion 4. transformation, 5. selection for antibiotic resistance Amp
2.
a- step 1 ; b- step 2 &3 ; c- step 5; d- step 4; e- amp and LB
3. transformation transduction conjugation.ransformation.
Transformation is the uptake of DNA from outside the cell. Only a single strand is taken up, through a special protein complex in the cell membrane. Phage Transduction
There are two types:
Conjugation
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