Antibody Staining Using antibody staining, you observe that your protein of inte

Question

Antibody Staining

Using antibody staining, you observe that your protein of interest localizes as shown in the picture to the right (the white regions).

1. What can you conclude about localization of the mRNA at this stage? (1 pt)

2. Give TWO reasons for this conclusion. (1 pt)

Antibody Staining Using antibody staining, you observe that your protein of interest localizes as shown in the picture to the right (the white regions). 1. What can you conclude about localization of the mRNA at this stage? (1 pt) 2. Give TWO reasons for this conclusion. (1 pt)

Explanation / Answer

1. Conclusion is that mRNA seems to be localized at poles of cells

2. Preparing to divide and an indicator of actin molecules being translated at site,

This is also seen in drosophilla during development stages.

mRNA starts its life as nascent transcripts that are first processed and then exported from the nucleus into the cytoplasm. Such transcripts associate in the nucleus with RNA binding proteins to form ribonucleoprotein complexes (RNPs), Specific RNA binding proteins within RNPs play essential roles in mRNA localization, translational regulation and degradation.

The given case seems to be of Positional Information where localization of mRNAs within a cell
- used only in cases where developmental field begins as a single cell
- used as a way of asymmetrically distributing local determinants to progeny cells

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