A purified DNA preparation of a certain plasmid is digested to completion with N
ID: 38712 • Letter: A
Question
A purified DNA preparation of a certain plasmid is digested to completion with NheI restriction endonuclease. In separate reactions, the same preparation was digested to completion with XbaI and with a mixture of XbaI and NheI, respectively. The diagram above shows the appearance of the original molecules and the digestion products in the three digestion mixtures, after separation by electrophoresis in an agarose gel. Was the original plasmid DNA circular or linear and What was the original DNA molecule's length?
Question options:
linear and 4 kb.
linear and 8 kb.
linear and 1.7 kb.
circular and 4 kb.
circular and 8 kb.
circular and 1.7 kb.
linear and 4 kb.
linear and 8 kb.
linear and 1.7 kb.
circular and 4 kb.
circular and 8 kb.
circular and 1.7 kb.
A purified DNA preparation of a certain plasmid is digested to completion with NheI restriction endonuclease. In separate reactions, the same preparation was digested to completion with XbaI and with a mixture of XbaI and NheI, respectively. The diagram above shows the appearance of the original molecules and the digestion products in the three digestion mixtures, after separation by electrophoresis in an agarose gel. Was the original plasmid DNA circular or linear and What was the original DNA molecule's length? Question options:Explanation / Answer
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