You isolate intact mitochondria and equilibrate them in a buffered solution at p

Question

You isolate intact mitochondria and equilibrate them in a buffered solution at ph 9 containing .1 KCL and ADP plus P, but without succinate ( oxidizable substrate). You collect them by centrifugation and quickly re suspend them in a new buffer at pH 7 without KCL but with valinomycin (a K+ iodophore) the K + rushing out will create a large positive charge differential. What will happen to oxygen consumption and the production of ATP? You isolate intact mitochondria and equilibrate them in a buffered solution at ph 9 containing .1 KCL and ADP plus P, but without succinate ( oxidizable substrate). You collect them by centrifugation and quickly re suspend them in a new buffer at pH 7 without KCL but with valinomycin (a K+ iodophore) the K + rushing out will create a large positive charge differential. What will happen to oxygen consumption and the production of ATP?

Explanation / Answer

In oxidative phosphorylation, the process is depends on the generation of "proton motive force". With the expulsion of more potassium ions, more hydrogen ions move inside the mitochondrial membrane by exchange, this creats a large proton gradient across the mitochondrial membrane. This would result in more oxygen consumption and more generation of ATP.

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