You injected a mouse with a protein of the salmonella outer membrane to have the

Question

You injected a mouse with a protein of the salmonella outer membrane to have the mouse make antibodies to this protein. To test the production of antibodies to the protein, you collect sera and incubate it with salmonella in an immunofluorescent assay to detect specific antibody. The assay works with a positive control serum but NOT with the sera from your mouse. In the sera from your mouse, there is no fluorescence and the salmonella are all dead with holes in them.

A. What can you conclude from these results? 4 pts

B. What specific initiation components would have caused the death of the salmonella- be specific! 4 pts

C. What would be 2 biologically active small molecules released during this process and what is a function of these small molecules? 8 pts

Explanation / Answer

Ans (A) : It can be concluded from the results that the sera of the injected mouse contains the antibiodies against salmonella which results into the death of the salmonella.

And (B) : complement system proteins formed that attacks the cell membrane of the salmonella which results into its death .

Ans (C) : antibodies and cytokines produced in this process. Antibodies activates the complement system which will result into the production of c3b proteins C5a proteins which will attack the salmonella cell membrane. Cytokines also results in the death of salmonella.

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