1. After heating the electrophoresis buffer with the agarose, the agarose dissol

Question

1. After heating the electrophoresis buffer with the agarose, the agarose dissolves. After cooling the hot liquid, a fluorescent dye needs to be added, so that the DNA can be visualized. The dye needs to be diluted 10,000-fold. How much dye does Chad need to add to his 30 mL gel solution?

300 uL

2. Chad has to make a 1.5% agarose gel so that he can analyze the products from his colony PCR. The total volume of the gel is 30 mL. How much agarose does he need to weigh out?

A. 3 mL B. 3 pL C. 3 nL D. 30 uL E. 3 uL F.

300 uL

2. Chad has to make a 1.5% agarose gel so that he can analyze the products from his colony PCR. The total volume of the gel is 30 mL. How much agarose does he need to weigh out?

A. 0.45 g B. 6 g C. 3 g D. 0.3 g E. 4.5 g F. 0.6 g

Explanation / Answer

1. 3 microliters. (1 *30 = x * 10,000; x= 0.003ml= 3 microliter)

2. He needs to add 1.5 gm agarose to 100ml distilled water. To make a 30 ml solution, the calculation will be -

       = (30*1.5) / 100 = 0.45 gm

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