Harry Potter cloned a human gene (Muggle1) into a bacterial expansion vector so

Question

Harry Potter cloned a human gene (Muggle1) into a bacterial expansion vector so that there is a myc tag. an eterokinase site, and a histidine tag at the C-Terminus (a myc tag is a small amino acid sequence that can illicit an immune response). After inducing expression and applying the crude vacterial extract over a NI+2-agrose column, he subjected the effluent to both SDS-PAGE/Coomasi Blue and Western Blot analysis. The SDS-PAGE gel shows a prominent band around 70kD with several intese bands at 45kD, 33kD, and 25kD.

What are the ebst antibodies to detect the recomibant Muggle1 protein while at the same time determine if the lower bands are products of degradtion at the N-Terminus as opposed to the co-purfiying contaminatens

Mark your selection in the table below.

If more than one antibody is used indicate the order of use (1,2,3, ect...)

Goat anti myc tag Fish anti-histidne tag Donkey anti-histidine tag Eterokinase anti-chicken IgG Donkey anti-myc tag Harry Potter cloned a human gene (Muggle1) into a bacterial expansion vector so that there is a myc tag. an eterokinase site, and a histidine tag at the C-Terminus (a myc tag is a small amino acid sequence that can illicit an immune response). After inducing expression and applying the crude vacterial extract over a NI+2-agrose column, he subjected the effluent to both SDS-PAGE/Coomasi Blue and Western Blot analysis. The SDS-PAGE gel shows a prominent band around 70kD with several intese bands at 45kD, 33kD, and 25kD. What are the ebst antibodies to detect the recomibant Muggle1 protein while at the same time determine if the lower bands are products of degradtion at the N-Terminus as opposed to the co-purfiying contaminatens Mark your selection in the table below. If more than one antibody is used indicate the order of use (1,2,3, ect...) Goat anti myc tag Fish anti-histidne tag Donkey anti-histidine tag Eterokinase anti-chicken IgG Donkey anti-myc tag Fish ant-Muggle1 Chicken anti-donkey IgG conjucated HRP Donkey anti-histidine conjugated HRP Goat anti-chicken IgG conjugated HRP Goat anti-donkey IgG

Explanation / Answer

Goat anti-myc tag----- Goat anti donkey Ig G

fish anti histidine tag----- Fish anti muggle 1

Donkey anti histidine tag- Donkey anti-histidine conjugated HRP

Enterokinase anti-chicken IgG---- Chicekn anti donkey IgG conjugated HRP

Donkey anti-myc tag--- Goat anti chicken IgG conjugated HRP

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