A sample of an unknown peptide undergoes a single round of Edman degradation, wh

Question

A sample of an unknown peptide undergoes a single round of Edman degradation, which liberates ATZ-Gly. Another sample of the peptide is treated with trypsin, and a third sample is treated with chymotrypsin. The table below shows the fragments. Complete parts (a) and (b) below.

a) Line up the fragments below so that fragments with the same sequences overlap. Drag each fragment to the appropriate location.

b) Write the sequence of the peptide.

A sample of an unknown peptide undergoes a single round of Edman degradation, which liberates ATZ-Gly. Another sample of the peptide is treated with trypsin, and a third sample is treated with chymotrypsin. The table below shows the fragments. Complete parts (a) and (b) below. a) Line up the fragments below so that fragments with the same sequences overlap. Drag each fragment to the appropriate location. b) Write the sequence of the peptide.

Explanation / Answer

Hi,

A) 1) Gly-Val-Tyr

2) Gly-Val-Tyr-Arg

3) Arg-Asn-Tyr

4) Asn-Tyr-Ala-Pro-Thr-Cys-Val-Gln-Lys

5) Ala-Pro-Thr-Cys-Val-Gln-Lys-Phe

6) Phe-Ser

7) Ser

B) the sequence of peptide should be

Gly-Val-Tyr-Arg-Asn-Tyr-Ala-Pro-Thr-Cys-Val-Gln-Lys-Phe-Ser

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