In this study yeast production in a stirred tank bioreactor using semi defined m

Question

In this study yeast production in a stirred tank bioreactor using semi defined medium containing glucose (90.0 g/I) as the main carbon source was evaluated. Experimental assays were performed according to a 2X2 full factorial design to evaluate the influence of aeration (0.25 to 0.75 wm) and agitation (150 to 250 rpm) conditions on yeast production AssayX,XProduction (g/I) Uniformity 1.8 1.3 1,-123.0, 23.2, 22.5 1,117.7, 18.0, 17.9 1,-126.7, 25.0, 27.0 2.3 1,1 16.2, 16.4, 15.9 4 1.3 e) Byhand, perform an ANOVA on the above data. dentify which factors are significant.Perform a t-test for each effect and each interaction. (b) Generate the 95% confidence interval for each effect and interaction. (c) In what direction does the optimal condition (maximum yeast production) reside? Estimate the optimal conditions for this process in both coded and uncoded units. (d) For a rotatable Central Composite Design, what should the value for a be? (e) Do a complete residual analysis

Explanation / Answer

Yeast Production Manual ( by hand): Glucose 90.0 grams per liter 2X2 full factorial: 2X2 = 4 hence 00, 01, 10, 11 that means, -1,-1; -1,1; 1,-1; 1,1 ( just a typical truth table for evaluation) Aeration effect: 0.25 to 0.75 wm Agitation effect: 150 to 250 revolutions per minute (rpm) 23 23.2 22.5 1.8 17.7 18 17.9 1.3 26.7 25 27 2.3 16.2 16.4 15.9 1.3

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