19) DNA sequencing was performed according to the dideoxynucleotide method. The

Question

19) DNA sequencing was performed according to the dideoxynucleotide method. The results of each one of the four reactions was loaded on a gel for electrophoresis. The picture of the resulting gel is shown (including the direction of DNA electrophoresis). Write the DNA sequence obtained. 20) Under ideal conditions, you would expect to end up with ______X more DNA after four PCR cycles. 21) Based on the genetic code table in chapter 7, can you predict if the LRNA carrying the amino acid tryptophan is capable of wobbling when base-pairing with the codon sequence. Explain your reasoning.

Explanation / Answer

Ans.

19.

TCCAACGT

20. 15x more DNA.
as, afterr 4 PCR cycles,
x = 2x = 4x = 8x = 16x
16x - x = 15 times more

21.

A Wobble Base Pair is a pairing between two nucleotides in RNA molecules that
does not follow Watson-Crick base pair rules.
The four main wobble base pairs are Guanine-Uracil (G-U),
Hypoxanthine-Uracil (I-U),
Hypoxanthine-Adenine (I-A)
   and Hypoxanthine-Cytosine (I-C)

Now Tryptophan is coded by codon UGG
so its anticodon should be, ACC
but now due to Wobbling,
it can be I/G/A U/C U/C (i.e, ACC or ACU or AUC or AUU or ICC or ICU or IUC or IUU or GCC or GCU or GUC or GUU)

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