22. You are using bacteriophages to map the genome of a new bacteria species and

Question

22. You are using bacteriophages to map the genome of a new bacteria species and find the locations of three toxin-production genes (R, Y, and G). You grow phages in a strain of the bacteria that produces all three toxins (R+, Y+, G+), isolate the phage, and then infect a second bacterial strain that cannot produce any of the toxins (R-, Y-, G-). The recipient bacteria are then grown on colorimetric media (media that changes color in response to toxin presence) to see which toxin genes are transferred together by the phage. The data are as follows: a. Which gene is in the middle? (3 points) b. Which two genes are closest to one another? (3 points) 23. How many of each of the following does this double-stranded DNA sequence have? A._ 3' hydroxyl (OH) groups (2 points) B.._ hydrogen bonds (2 points) C.._ pyrimidines (2 points) D.. _phosphodiester bonds (2 points)

Explanation / Answer

22. a .. G is in the middle

b.. G and R

23.A. 2

B.51(2 bonds between Adenine and thymine; 3 bonds between cytosine and guanine)

C.16

D. 38

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