Experiment 1: Ecological Interactions Procedure 1. Measure out 990 mL of water i

Question

Experiment 1: Ecological Interactions Procedure 1. Measure out 990 mL of water into your big jug using the 100 mL graduated cylinder. The jug represents the primary producers. Measure 10 mL of oil and add it to the jug with 990 mL. Clean the 10 mL graduated cylinder so there is no oil residue. 4 Let the oil coalesce at the top of the container, the oil is the accumulation agent. It is insoluble because we want it to represent the organism's inability to break it down. s Calculate the volume of water and the volume of oil in your 1000 mL container. Calculate percent concentration of oil. Record in Table I. HS: V (of two or more substances) (V (of single substance)! total V of the mixture) x 100 * Pour 100 mL of the mixture into the 100 mL graduated cylinder and let the mixture settle. , Calculate the volume of water and the volume of oil in your 100 mL container. Calculate the percent concentration of oil. Record in Table I. Now. pour 10 mL of the mixture into the 10 mL graduated cylinder. $ Calculate the volume of water and the volume of oil in the 10 mL graduated cylinder. Calculate the percent concentration of oil. calculate the volume or water and the volume of on in the 10 mL graduated cylinder. Calculate the percent concentration of oil. Post-Lab Questions 1. What is the percent concentration of oil in the first, second, and third trophic levels in our food chain? How did the concentration of oil change from one trophic level to the next? 3. How does the change in concentration represent biomagnification? 4. How does this also illustrate the amount of energy transferred from one trophic level to the next? If you would like to read more about issues surrounding bioaccumulation simply search for case studies that address POP (persistent organic pollutants) and biomagnification in ecosystems.

Explanation / Answer

% Concentration of Oil= (Volume of Oil/Total Volume) x 100

Tropic level

Cylinder

Volume of H2O

Volume of Oil

Total Volume

Percent oil

1

1000mL

990mL

10mL

1000mL

1%

2

100mL

97mL

3mL

100mL

3%

3

10mL

9.5mL

5mL

10mL

5%

1) What is the percent concentration of oil in the first, second and third tropic levels in our food chain?

Answer) In first level, the oil percent concentration is 1%, In second level it is 3% and in third tropic level it is 5%.

2) How did the concentration of oil change from one trophic level to the next?

Answer) As you see in the above table, the concentration of oil has gradually increased and became more dense from one trophic level to the next.

3) How does the change in concentration represent bio magnification?

Answer) The above raise of oil represent bio magnification, as the oil started accumulating in the process and it has raised from 1% to 5%. In the same way, accumulation of other things can also be observed as we go up in trophic levels. Increase in concentration can be related with the biomaganification.

4) How does this also illustrate the amount of energy transferred from one tropic level to the next?

Answer) We can say there will be a loss of energy as we move up the trophic levels as the energy is lost as metabolic hear ( organisms from one tropic level are consumed by organisms from the next level), as we can see the amount of oil transferred is becoming lesser.

Tropic level

Cylinder

Volume of H2O

Volume of Oil

Total Volume

Percent oil

1

1000mL

990mL

10mL

1000mL

1%

2

100mL

97mL

3mL

100mL

3%

3

10mL

9.5mL

5mL

10mL

5%

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