6. You are interested in how proteins are imported into and eported out of the n

Question

6. You are interested in how proteins are imported into and eported out of the nucleus. For your experiment you use several mutant cell lines or treat normal cells with abnormal conditions. You examine 2 different proteins - protein A is 100 kD and protein is 25 kD. Both proteins have both an NLS and an NES. Neither NLS or NES is ever masked.

a) In Mutant 2 you can inactivate all NXF1 and NXT1 so that they have none of their normal functions. You induce this mutant to start translating protein B and inactivate NXF1 and NXT1 simultaneously. Where will protein B be found in these cells? EXPLAIN

Explanation / Answer

Since the protein of interest protein B is being translated which has both NLS and NES. Therefore, protein B will be found in the cell nucleus. Reason being that both NXF1 and NXT1 which are export proteins (that act as nuclear export factor) are inactivated by the mutant while protein B is translating.

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