The images below is DNA sequence (in reading frame) and the amino acid translati
ID: 36139 • Letter: T
Question
The images below is DNA sequence (in reading frame) and the amino acid translation for a region of a protein coding gene for Iwo species you are researching. By comparing the two alignments and/or using she translation table provided (remember U mark all of the mutations in the DNA alignment as either non-synonymous (N) or synonymous (S). Place either an W or an S in the space above the mutation in the DNA alignment. b. How many non-synonymous mutations (N) are there? c. How many synonymous mutations (S) are there? d. What is the dN/dS? How is this different than the Ka/Ks ratio? e. If the value from part d. above was an adusted Ka/Ks ratio. would you estimate the gene had been under selection in these lineages or has it been evolving neutrally? If it is under selection. what type of selection pressure is at work?Explanation / Answer
A) In the order, N, N, S, N, N, N, S, N, (N/N), N, N, --, -- B) 9 Nonsynonymous (Dn) mutations C) 2 Synonymous (Ds) mutations D) Both are indicators of selective pressure in evolution, dN/dS (calculate at sequence level), Ka/Ks (only calculate at protein coding regions), dN/dS= 9/2= 4.5 E) Yes, positive or Darwinian selection, since the Ka/Ks ratio is >1.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.