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I am trying to build a small horizontal electrophoresis chamber from scratch. I

ID: 35496 • Letter: I

Question

I am trying to build a small horizontal electrophoresis chamber from scratch. I want to use it for comet assay and I will be using only 1 slide, so it's going to be about 3cm wide, 10cm long and 4cm high, just enough to fit the slide and a small amount of buffer. As far as I know such thing is not commercially available, so before I make it I want to make sure that it's going to work. The main issue is the cost of the electrode (has to be platinum wire). I don't want to spent that much money just to realize that it doesn't work. My question is: is a 3cm electrode in a 60-70cm3 enough? Basically, are there any physical restrictions on the size and the position of the electrodes and the shape of the chamber? Since I am a biologist and my knowledge in physics are quite limited, I would greatly appreciate any information about this.

Explanation / Answer

These instructions for building an electrophoresis chamber don't comment on the minimum length of wire, but on the other hand they suggest using something called Monel Seizing Wire (Ni/Cu alloy -

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