Example #1: Given that the membrane is impermeable to NaCl but permeable to wate

Question

Example #1: Given that the membrane is impermeable to NaCl but permeable to water

a. What is the osmolarity of 0.5 Osm NaCl relative to the RBC?

i. Before blood is added to the solution?

ii. After blood is added to the solution?

b. What is the appearance of 0.5 Osm NaCl solution hen blood is added?

c. What is the appearance of the RBC when placed in 0.5 osm NaCl?

Example #2: Given that the membrane is permeable to both Urea and Water.

What is the osmolarity of 0.5 Osm NaCl relative to the RBC?

i. Before blood is added to the solution?

ii. After blood is added to the solution?

b. What is the appearance of 0.5 Osm NaCl solution hen blood is added?

c. What is the appearance of the RBC when placed in 0.5 osm NaCl?

Explanation / Answer

a)i)Before blood is added to the solution, the Normal saline solution osmolarity is an in close approximation to the osmolarity of NaCl in the blood.

ii) When blood is added to the 0.5% NaCl solution, the RBC's present in the blood will swell, it happens due to the movement of water inside it resulting due to the phenomenon of osmosis. It will lead to bursting of the cell. ii) Similarly, RBC's when placed in hypertonic solution, the shrinking of the cell will occur due to the water movement out of the cell, and shrinking of RBC's takes place due to the plasmolysis.

b)We can say the 0.5% NaCl solution is isotonic in nature and when the blood cells added in such a medium, there will be the osmotic equilibrium between intracellular and extracellular fluids across the cell membrane, and net influx or efflux of water will not take place

c)0.5% NaCl is an isotonic solution it will not damage the blood cells or tissues, and there will be a drive of water into the tissues if the solution is hypotonic.

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